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Pipet 10 mL of the substrate solution, stand at 37±0.50 for 10 minutes, add exactly 1 mL of the sample solu-tion, and shake immediately. Allow this solution to stand at37±0.5 C for exactly 10 minutes, add exactly 2 mL of alkaline tartrate solution of the Fehling's TS for amylolytic activity test, and shake immediately. Then, add exactly 2 mL of copper solution of the Fehling's TS for amylolytic activity# ^8 m: Q I3 b. V% A
test, shake gently, heat the solution in a water bath for ex-actly 15 minutes, and then immediately cool to below 25 C.Then, add exactly 2 mL of concentrated potassium iodide TS and2mLofdilutedsulfuricacid(1in6), andtitrat the released iodine with 0.05 mol/L sodium thiosulfate VS to the disappearance of the blue color produced by addition of 1 to 2 drops of soluble starch TS (amL). Separately, pipet 10 mL of water instead of the substrate solution and titrate <2.50>inthesamemanner(bmL).
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请教一下,文中heat the solution in a water bath for eactly 15 minutes, 水浴加热中水浴的温度应该为多少呢?; H# U {& L& ]* e5 A
实在搞不明白,麻烦大侠们告诉一下喽!4 X7 X& C) v& S1 G- h" q$ @3 b! Q% J
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