马上注册,结交更多好友,享用更多功能,让你轻松玩转社区
您需要 登录 才可以下载或查看,没有帐号?立即注册
x
Pipet 10 mL of the substrate solution, stand at 37±0.50 for 10 minutes, add exactly 1 mL of the sample solu-tion, and shake immediately. Allow this solution to stand at37±0.5 C for exactly 10 minutes, add exactly 2 mL of alkaline tartrate solution of the Fehling's TS for amylolytic activity test, and shake immediately. Then, add exactly 2 mL of copper solution of the Fehling's TS for amylolytic activity
0 E3 G* J3 T% y) S% _6 j. u3 V- [/ itest, shake gently, heat the solution in a water bath for ex-actly 15 minutes, and then immediately cool to below 25 C.Then, add exactly 2 mL of concentrated potassium iodide TS and2mLofdilutedsulfuricacid(1in6), andtitrat the released iodine with 0.05 mol/L sodium thiosulfate VS to the disappearance of the blue color produced by addition of 1 to 2 drops of soluble starch TS (amL). Separately, pipet 10 mL of water instead of the substrate solution and titrate <2.50>inthesamemanner(bmL).
7 |* b" P( O/ G
4 l( x" C& |! x( j2 P( w' {, F9 B4 Y' p
请教一下,文中heat the solution in a water bath for eactly 15 minutes, 水浴加热中水浴的温度应该为多少呢?
! A% l* k, J: s7 i4 a' Y实在搞不明白,麻烦大侠们告诉一下喽!3 O0 c! U7 H% y# v
|